Gruaduate School of Veterinary Medicine

The laboratory'sAchievements

2023年

Original papers

1)Lau, A. C. C., Mohamed, W. M. A., Nakao, R., Onuma, M., Qiu,Y., Nakajima, N., Shimozuru, M., Mohd-Azlan, J., Moustafa, M. A. M. & Tsubota, T.: The dynamics of the microbiome in Ixodidae are shaped by tick ontogeny and pathogens in Sarawak, Malaysian Borneo. Microbial Genomics 9(2): 000954, 2023.

2)坪田敏男:クマ類の冬眠−繁殖との関係−.低温科学 81:173-180, 2023.

3)下鶴倫人:クマの冬眠の生理・代謝機構~如何に太り、如何に痩せるか~.低温科学 81:181-189, 2023.

4)Nakamura, S., Yamazaki, J., Matsumoto, N., Inoue-Murayama, M., Qi, H., Yamanaka, M., Nakanishi, M., Yanagawa, Y., Sashika, M., Tsubota, T., Ito, H. & Shimozuru, M.: Age estimation based on blood DNA methylation levels in brown bears. Molecular Ecology Resources, 23:1211-1225, 2023.

5)Ghimirey, Y., Acharya, R., Yadav, K., Rai, J. Baral, R., Neupane, U., Basnet, B., Gilbert, M., Poudel, S. & Rensburg, B. van: 2024. Challenges and possible conservation implications of recolonizing dholes Cuon alpinus in Nepal. Oryx. Published online 1-9, 2024.

6)Malla, R., Panthi, S., Adhikari, H., Pariyar, S., Baral, R., Subedi, R., Adhikari, B. P., Poudel, M., Sedhai, N., Poudel, M.: Habitat suitability of four threatened Himalayan species: Asiatic black bear, common leopard, musk deer, and snow leopard. PeerJ 11: e16085, 2023.

7)Baral, R., Gurung, D. K. & Som G.C.: High altitude records of Orange-headed Thrush Geokichla citrina and Asian Koel Eudynamys scolopaceus in the Nepal Himalaya. Indian BIRDS 19: 20-21, 2023.

 

 

2022年

Original papers

1)Miyazaki, M., Shimozuru, M. & Tsubota, T.: Supplementing cultured human myotubes with hibernating bear serum results in increased protein content by modulating Akt/FOXO3a signaling. PLoS ONE 17(1): e0263085, 2022.

2)Takaesu, N., Kanno, C., Sugimoto, K., Nagano, M., Kaneko, A., Indo, Y., Imai, H., Hirai, H., Okamoto, M., Sashika, M., Shimozuru, M., Katagiri, S., Tsubota, T. & Yanagawa, Y.: Semen collection by urethral catheterization and electro-ejaculation with different voltage, and effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque. J. Vet. Med. Sci. 84(3): 429-438, 2022.

3)Takaesu, N., Minami, M., Nagano, M., Kaneko, A., Indo, Y., Imai, H., Hirai, H., Sashika, M., Shimozuru, M., Okamoto, M., Katagiri, S., Tsubota, T. & Yanagawa, Y.: Ovulatory follicle size investigated by ultrasonography and single artificial insemination with ovulation induction in the Japanese macaque. Jpn. J. Vet. Res. 70(1): 5-17, 2022.

4)Paudel, R. P., Kadariya, R., Lamichhane, B. R., Subedi, N., Sashika, M., Shimozuru, M. & Tsubota, T.: Habitat occupancy of sloth bear Melursus ursinus in Chitwan National Park, Nepal. Ecology and Evolution 12: e8699, 2022.

5)Jimbo, M., Ishinazaka, T., Shirane, Y., Umemura, Y., Yamanaka, M., Uno, H., Sashika, M., Tsubota, T. & Shimozuru, M.: Natal habitat influence on lifelong foraging strategies in solitary large mammals. Ecosphere 13: e4105, 2022.

6)Uraguchi, K., Irie, T., Kouguchi, H., Inamori, A., Sashika, M., Shimozuru, M., Tsubota, T. & Yagi, K.: Anthelmintic baiting of foxes against Echinococcus muttilocularis in small public area, Japan. Emerg. Inf. Dis. 28(8): 1677-1680, 2022.

7)Kawamura,K., Jimbo, M., Adachi, K., Shirane, Y., Nakanishi, M., Umemura, Y., Ishinazaka, T., Uno, H., Sashika, M., Tsubota, T. & Shimozuru, M.: Diel and monthly activity pattern of brown bears and sika deer in the Shiretoko Peninsula, Hokkaido, Japan. J. Vet. Med. Sci. 84(8): 1146-1156, 2022.

8)Shimozuru, M., Jimbo, M., Adachi, K., Kawamura, K., Shirane, Y., Umemura, Y., Ishinazaka, T., Nakanishi, M., Kiyonari, M., Yamanaka, M., Amagai, Y., Ijuin, A., Sakiyama, T., Kasai, S., Nose, T., Shirayanagi, M., Tsuruga, H., Mano, T., Tsubota, T., Fukasawa, K. & Uno, H.: Estimation of breeding population size using DNA-based pedigree reconstruction in brown bears. Ecology and Evolution 12: e9246, 2022.

9)Lau, A. C. C., Qiu, Y., Moustafa, MAM, Nakao, R., Shimozuru, M., Onuma, M., Nakajima, N., Sashika, M., Mohd-Azlan, J. & Tsubota, T.: Detection of a Babesia sp. Genotype closely related to marsupial-associated Babesia spp. in male Haemaphysalis shimoga from Sarawak, Malaysian Borneo. J. Vet. Med. Sci. 84(11): 1469-1473, 2022.

10) Miyazaki, M., Shimozuru, M., Kitaoka, Y., Takahashi, K. & Tsubota, T.: Regulation of protein and oxidative energy metabolism are down-regulated in the skeletal muscles of Asiatic black bears during hibernation. Scientific Reports 12, 19723, 2022.

 

 

2021年

Original papers

1) Nishida, K., Shimozuru, M., Okamatsu-Ogura, Y., Miyazaki, M., Soma, T., Sashika, M. & Tsubota, T.: Changes in liver microRNA expression and their possible regulatory role in energy metabolism-related genes in hibernating black bears. J. Comp. Physiol. B 191(2): 397-409. 2021.

2) Shirane, Y., Jimbo, M., Yamanaka, M., Nakanishi, M., Mori, F., Ishinazaka, T., Sashika, M., Tsubota, T. and Shimozuru, M.: Dining from the coast to the summit: Salmon and pine nuts determine the summer body condition of female brown bears on the Shiretoko Peninsula. Ecology and Evolution 11: 5204-5219, 2021.

3) Moustafa, M. A. M., Anders, J. L., Mohamed, W. M. A., Lee, K., Elbaz, E., Sashika, M., Nakao, R., Shimozuru, M. & Tsubota, T.: Specific molecular detection of piroplasms and characterization of β-tubulin for a novel babesia species in sika deer (Cervus nippon yesoensis). J. Zoo Wildl. Med. 52(1): 200-205, 2021.

4) Paudel, S., Brenner, E., Hadi, S., Suzuki, Y., Nakajima, C., Tsubota, T., Gairhe, K., Maharjan, B. & Sreevatsan, S.: Genome Sequences of two Mycobacterium tuberculosis Isolates from Asian Elephants of Nepal. Microbiology Resource Announcements 10(36): e00614-21, 2021.

5) Miyazaki, M., Shimozuru, M. & Tsubota, T.: Supplementing cultured human myotubes with hibernating bear serum results in increased protein content by modulating Akt/FOXO3a signaling. PLoS ONE 17(1): e0263085, 2022.

6) Takaesu, N., Kanno, C., Sugimoto, K., Nagano, M., Kaneko, A., Indo, Y., Imai, H., Hirai, H., Okamoto, M., Sashika, M., Shimozuru, M., Katagiri, S., Tsubota, T. & Yanagawa, Y.: Semen collection by urethral catheterization and electro-ejaculation with different voltage, and effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque. J. Vet. Med. Sci. 84(3): 429-438, 2022.

7) Takaesu, N., Minami, M., Nagano, M., Kaneko, A., Indo, Y., Imai, H., Hirai, H., Sashika, M., Shimozuru, M., Okamoto, M., Katagiri, S., Tsubota, T. & Yanagawa, Y.: Ovulatory follicle size investigated by ultrasonography and single artificial insemination with ovulation induction in the Japanese macaque. Jpn. J. Vet. Res. 70(1): 5-17, 2022.

8) Paudel, R. P., Kadariya, R., Lamichhane, B. R., Subedi, N., Sashika, M., Shimozuru, M. & Tsubota, T.: Habitat occupancy of sloth bear Melursus ursinus in Chitwan National Park, Nepal. Ecology and Evolution 12: e8699, 2022.

9) Jimbo, M., Ishinazaka, T., Shirane, Y., Umemura, Y., Yamanaka, M., Uno, H., Sashika, M., Tsubota, T. & Shimozuru, M.: Natal habitat influence on lifelong foraging strategies in solitary large mammals. Ecosphere, in press.

10) Kodama, F., Yamaguchi, H., Park, E., Tatemoto, K., Sashika, M., Nakao, R., Terauchi, Y., Mizuma, K., Orba, Y., Kariwa, H., Hagiwara, K., Okazaki, K., Goto, A., Komagome, R., Miyoshi, M., Ito, T., Yamano, K., Yoshii, K., Funaki, C., Ishizuka, M.,Shigeno, A., Itakura, Y., Bell-Sakyi, L., Edagawa, S., Nagasaka, A., Sakoda, Y., Sawa, H., Maeda, K., Saijo, M., Matsuno, K.:A novel nairovirus associated with acute febrile illness in Hokkaido,Japan. Nat. Commun. 12(1):5539, 2021.

 

 

2020年

Original papers

Shimozuru, M., Shirane, Y., Jimbo, M., Yamanaka, M., Nakanishi, M., Ishinazaka, T., Kasai, S., Nose, T., Fujimoto, Y., Tsuruga, H., Mano, T. & Tsubota, T.: Male reproductive input, breeding tenure, and turnover in high-density brown bear (Ursus arctos yesoensis) populations on the Shiretoko Penninsula, Hokkaido, Japan. Can. J. Zool. 98(3): 175-185, 2020. Doi.org/10.1139/cjz-2019-0061

Okuyama, M. W., Shimozuru, M., Nakai, M., Yamaguchi, E., Fujii, K., Shimada, K., Ikeda, T. & Tsubota, T.: Genetic population structure of invasive raccoons (Procyon lotor) in Hokkaido, Japan: Unique phenomenon caused by pet escape or abandonment. Sci. Rep. 10, 8108, 2020. Doi.org/10.1038/s41598-020-64526-y

Nurliani, A., Sasaki, M., Budipitojo, T., Tsubota, T. & Kitamura, N.: Morphological and histological studies on the epididymis and deferent duct of the Sunda porcupine (Hystrix javanica). Mammal Study 45(3): 201-211, 2020. Doi.org/10.3106/ms2019-0061

Nasoori, A., Okamatsu-Ogura, Y., Shimozuru, M., Sashika, M. & Tsubota, T.: Hibernating bear serum hinders osteoclastogenesis in-vitro. PLoS ONE 15(8): e0238132, 2020. Doi.org/10.1371/journal.pone.0238132

Moustafa, M. A. M., Sasaki,S., Shimozuru, M., Nakao, R., Sashika, M., Yamazaki, K., Koike, S., Tanaka, J., Tamatani, H., Yamanaka, M., Ishinazaka, T. & Tsubota, T.: Molecular detection of apicomplexan protozoa in Hokkaido brown bears (Ursus arctos yesoensis) and Japanese black bears (Ursus thibetanus japonicus). Parasitology Research 119(11): 3739-3753, 2020. Doi.org/10.1007/s00436-020-06873-3

Jimbo, M., Matsumoto, N., Sakamoto, H., Yanagawa, Y., Torii, Y., Yamanaka, M., Ishinazaka, T., Shirane, Y., Sashika, M., Tsubota, T. & Shimozuru, M.: Hair growth in brown bears and its application to ecological studies on wild bears. Mammal Study 45: 337-345, 2020. Doi.org/10.3106/ms2020-0021

Shirane, Y., Mori, F., Yamanaka, M., Nakanishi, M., Ishinazaka, T., Mano, T., Jimbo, M., Sashika, M., Tsubota, T. & Shimozuru, M.: Development of a noninvasive photograph-based method for the evaluation of body condition in free-ranging brown bears. PeerJ 8: e9982, 2020. Doi.org/10.7717/peerj.9982

Shimozuru, M., Shirane, Y., Yamanaka, M., Nakanishi, M., Ishinazaka, T., Kasai, S., Nose, T., Shirayanagi, M., Jimbo, M., Tsuruga, H., Mano, T. & Tsubota, T.: Maternal human habituation enhances son's risk of huma-caused mortality in a large carnivore, brown bears. Sci. Rep. 10, 16498, 2020. Doi.org/10.1038/s41598-020-73057-5

Elbaz, E., Moustafa, M. A. M., Lee, K., Lau, A. C. C., Shimozuru, M., Sashika, M., Nakao, R., El-khodery, S. A. & Tsubota, T: Utilizing the attached hard ticks as pointers to the risk of infection by Babesia and Theileria species in sika deer (Cervus nippon yesoensis), Japan. Experimental and Applied Acarology, 82: 411-429, 2020. Doi.org/10.1007/s10493-020-00551-w

Lau, C. C. A., Qiu, Y., Moustafa, M. A. M., Nakao, R., Shimozuru, M., Onuma, M., Mohd-Azlan, J. & Tsubota, T.: Detection of Borrelia burgdorferi Sensu lato and relapsing fever Borrelia in feeding Ixodes Ticks and rodents in Sarawak, Malaysia: New geographical records of Borrelia yangtzensis and Borrelia miyamotoi. Pathogens 9: 846-860, 2020. Doi.org/10.3390/pathogens9100846

Oe, S., Sashika, M., Fujimoto, A., Shimozuru, M. & Tsubota, T.: Predation impacts of invasive racoons on rare native species. Sci. Rep.10: 20860, 2020. Doi.org/10.1038/s41598-020-77016-y

 

 

2019年

Original papers

Paudel, S., Nakajima, C., Mikota, S., Gairhe, K., Maharajan, B., Subedi, S., Poudel, A., Sashika, M., Shimozuru, M., Suzuki, Y. & Tsubota, T.: Mixed mycobacterium tuberculosis lineage infection in two elephants, Nepal. Emerg. Inf. Dis. 25(5): 1031-1032, 2019.

Shimozuru, M., Shirane, Y., Tsuruga, H., Yamanaka, M., Nakanishi, M., Ishinazaka, T., Kasai, S., Nose, T., Masuda, Y., Fujimoto, Y., Mano, T. & Tsubota, T.: Incidence of multiple paternity and inbreeding in high-density brown bear populations on Shiretoko Peninsula, Hokkaido, Japan. J. Heredity 110(3): 321-331, 2019. doi: 10.1093/jhered/esz002

Fuchs, B., Yamazaki, K., Evans, A. L., Tsubota, T., Koike, S., Naganuma, T. & Arnemo, J. M.: Heart rate during hyperphagia differs between two bear species. Biol. Lett. 15: 20180681, 2019. http:://dx.doi.org/10.1098/rsbl.2018.0681

Paudel, S., Mikota, S. K. & Tsubota, T.: Tuberculosis threat in Asian elephants. Science 363(6425), 356, 2019. DOI: 10.1126/science.aaw2342

Miyazaki, M., Shimozuru, M. & Tsubota, T.: Skeletal muscles of hibernating black bears show minimal atrophy and phenotype shifting despite prolonged physical inactiveity and starvation. PLoS ONE 14(4): e0215489, 2019.

Shirane, Y.(*), Shimozuru, M. (*共同筆頭著者), Yamanaka, M., Tsuruga, H., Nakanishi, M., Ishinazaka, T., Nose, T., Kasai, S., Shirayanagi, M., Masuda, Y., Fujimoto, Y., Mano, T., Sashika, M. & Tsubota, T.: Sex-biased dispersal and inbreeding avoidance in Hokkaido brown bears. J. Mammal. 100(4): 1317-1326, 2019.

Paudel, S., Tsubota, T. & Mikota, S. K.: Human TB threat to elephants. Nature 571(7764): 174, 2019.

Osaki, A. (*), Sashika, M. (*共同筆頭著者), Abe, G., Shinjo, K., Fujimoto, A., Nakai, M., Shimozuru, M. and Tsubota, T.: Comparison of feeding habits and habitat use between invasive raccoons and native raccoon dogs in Hokkaido, Japan. BMC Ecology 19: 35-49, 2019.

Nuriani, A., Sasaki, M., Budipitogo, T., Tsubota, T., Suzuki, M. & Kitamura, N.: An immunohistochemical study on testicular steroidogenesis in the Sunda porculine (Histrix javanica). J. Vet. Med. Sci. 81(9): 1285-1290, 2019.

 

 

2018年

Original papers

Tomiyasu. J., Yanagawa. Y., Sato. Y., Shimozuru. M., Nagano. M., Sasaki. M., Sakamoto, H., Matsumoto. N., Kobayashi. K., Kayano. M., Haneda. S. & Matsui, M.: Testosterone-related and seasonal changes in sebaceous gland in the back skin of adult male brown bears (Ursus arctos). Can. J. Zool. 96(3):205-211, 2018.

Moriwaki, J., Omori, R., Shimozuru, M., Tsuruga, H., Mano, T. & Tsubota, T. 2018. Evaluation of body condition using body mass and chest girth in brown bears of Hokkaido, Japan (Ursus arctos yesoensis). Jpn. J. Vet. Res. 66(2): 71-81, 2018.

Shirane, Y., Shimozuru, M., Yamanaka, M., Tsuruga, H., Hirano, S., Nagano, N., Moriwaki, J., Nakanishi, M., Ishinazaka, T., Nose, T., Kasai, S., Shirayanagi, M., Masuda, Y., Fujimoto, Y., Osada, M., Akaishi, M., Mano, M., Masuda, R., Sashika, M. & Tsubota, T.: Sex-biased natal dispersal in Hokkaido brown bears revealed through mitochondrial DNA analysis. Eur. J. Wildl. Res. 64(6): 65, 2018.

Kadariya, R., Shimozuru, M., Maldonado, J. E., Moustafa, M. A. M., Sashika, M. & Tsubota, T.: High genetic diversity and distinct ancient lineage of Asiatic black bears revealed by non-invasive surveys in the Annapurna Conservation Area, Nepal. PLos ONE 13(12): e0207662, 2018.

Paudel, S., Mikota, S. K., Thapa, J., Lyashchenko, K. P., Gairhe, K. P., Dhakal, I. P., Subedi, N., Maharjan, B., Subedi, S., Kaufman, G. E. & Tsubota, T.: Serodiagnosis of elephant tuberculosis: A useful tool for early identification of infected elephants at the captive-wild interface. Eur. J. Wildl. Res. 64(6): 70-75, 2018.

Hong, Y., Kim, K.S., Kimura, J., Kauhala, K., Voloshina, I., Goncharuk, M.S., Yu, L., Zhang, Y.P., Sashika, M., Lee, H. & Min, M. S.: Genetic Diversity and Population Structure of East Asian Raccoon Dog (Nyctereutes procyonoides): Genetic Features in Central and Marginal Populations. Zoolog. Sci. 35(3):249-259, 2018.

 

 

2017年

Original papers

Moustafa, M. A. M., Shimozuru, M., Mohamed, W., Taylor, K. R., Nakao, R., Sashika, M. & Tsubota, T.: First molecular detection and characterization of Hepatozoon and Sarcosystis spp. in field mice and voles from Japan. Parasitology Research 116: 2321-2325, 2017. DOI 10.1007/s00436-017-5505-z

Shimozuru, M., Yamanaka, M., Nakanishi, M., Moriwaki, J., Mori, F., Tsujino, M., Shirane, Y., Ishinazaka, T., Kasai, S., Nose, T., Masuda, Y. & Tsubota, T.: Reproductive parameters and cub survival of brown bears in the Rusha area of the Shiretoko peninsula, Hokkaido, Japan. PLoS ONE 12(4): e0176251, 2017. https://doi.org/10.1371/journal.pone.0176251

Elbaz, E., Moustafa, M. A. M., Lee, K., Mohamed, W. M. A., Nakao, R., Shimozuru, M., Sashika, M., Younis, E. E. A., El-khodery, S. A. & Tsubota, T.: Molecular identification and characterization of piroplasm species in Hokkaido sika deer (Cervus Nippon yesoensis), Japan. Tick and Tick-borne Diseases 8: 802-807, 2017. Https://doi.org/10.1016/j.ttbdis.2017.06.007

下鶴倫人、山中正実:知床半島先端部地区におけるヒグマ個体群の保護管理、および、羅臼町住民生活圏へ与える影響に関する研究、知床博物館研究報告特別号2: 95-120, 2017.

 

 

2016年

Original papers

Fukui, D., Nakamura, M., Yamaguchi, T., Takenaka, M., Murakami, M., Yanai, T., Fukushi, H., Yanagida, K., Bando, G., Matsuno, K., Nagano, M. & Tsubota, T.: An epizootic of emerging novel avian pox in Carrion Crows (Corvus corone) and Large-billed Crows (Corvus macrorhynchos) in Japan. J. Wildl. Dis. 52(2): 230-241, 2016.

Thapa, J., Paudel, S., Shah, Y., Maharjan, B., Sadaula, A., Kaufman, G., McCauley, D., Gairhe, K. P., Tsubota, T., Suzuki, Y. & Nakajima, C.: Tuberculosis by Mycobacterium orgyis in wild rhinoceros of Nepal: challenges and new scenario of tuberculosis in South Asia. Emerg. Infect. Dis. 22(3): 570-572, 2016.

Shimozuru, M., Nagashima, A., Tanaka, J. & Tsubota, T.: Seasonal changes in the expression of energy metabolism-related genes in white adipose tissue and skeletal muscle in female Japanese black bears. Comparative Biochemistry and Physiology, Part B 196-197, 38-47, 2016.

Paudel, S., Villanueva, M. A., Mikota, S. K., Nakajima, C., Gairhe, K. P., Subedi, S., Rayamajhi, N., Sashika, M., Shimozuru, M., Matsuba, T., Suzuki, Y. & Tsubota, T.: Development and evaluation of an interferon-γ release assay in Asian elephants (Elephas maximus) . J. Vet. Med. Sci. 78(7): 1117-1121, 2016.

Paudel, S. & Tsubota, T.: Tuberculosis in elephants: A zoonotic disease at the human-elephant interface. Jpn. J. Zoo Wildl. Med. 21(3): 65-69, 2016.

坪田敏男:北海道の野生動物におけるマダニ媒介性感染症の宿主−ベクター−病原体の関係.Jpn. J. Zoo Wildl. Med. 21(3): 47-51, 2016.

Moustafa, M. A. M., Taylor, K., Nakao, R., Shimozuru, M., Sashika, M., Rosà, R., Thu, M., Rizzoli, A., Tsubota, T.: Dynamics, co-infections and characteristics of zoonotic tick-borne pathogens in Hokkaido small mammals, Japan. Ticks and Tick-Borne Diseases 7(5): 922-928, 2016.

Paudel, S., Brown, J. L., Thapaliya, S., Dhakal, I. P., Mikota, S. K., Kamal P. Gairhe, K. P., Shimozuru, M. & Tsubota, T.: Comparison of cortisol and thyroid hormones between tuberculosis-suspects and healthy elephants of Nepal. J. Vet. Med. Sci. 78(11): 1713-1716, 2016.

Nasoori, A., Shahbazzadeh, D., Tsubota, T. & Young, B.: The defensive behaviour of Naja oxiana, with comments on the visual displays of cobras. The Herpetological Bulletin 138: 13-17, 2016.

Moriwaki, J., Shimozuru, M., Tsuruga, H., Mano, T. & Tsubota, T.: Estimation of reproductive parameters and their annual variation in brown bears of Hokkaido, Japan. Ursus 27(2): 99-109, 2016.

 

 

2015年

Original papers

Yanagawa, Y., Matsuura, Y., Suzuki, M., Saga, S., Okuyama, H., Fukui, D., Bando, G., Nagano, M., Katagiri, S., Takahashi, Y. & Tsubota, T.: Accessory corpora lutea formation in pregnant Hokkaido sika deer (Cervus nippon) investigated by examination of ovarian dynamics and steroid hormone concentrations. J. Reprod. Dev. 61: 61-66, 2015.

坪田敏男:One Healthと保全医学−日本野生動物医学会20年のあゆみ−.日本野生動物医学会誌 20(2): 27-33, 2015.

Moustafa, M. A. M., Lee, K., Taylor, K. R., Nakata, R., Sashika, M., Shimozuru, M. & Tsubota, T.: Molecular characterization and specific detection of Anaplasma species (AP-sd) in sika deer and its first detection in wild brown bears and rodents in Hokkaido, Japan. Infection, Genetics and Evolution 36: 268-274, 2015.

平川浩文, 木下豪太, 坂田大輔, 村上隆広, 車田利夫, 浦口宏二, 阿部 豪, 佐鹿万里子. : 拡大・縮小はどこまで進んだか―北海道における在来種クロテンと外来種ニホンテンの分布―. 哺乳類科学. 55(2): 155-166, 2015.

 

 

2014年

Original papers

Paudel, S., Mikota, S. K., Nakajima, C., Gairhe, K. P., Maharjan, B., Thapa, J., Poudel, A., Shimozuru, M., Suzuki, Y. and Tsubota, T.: Molecular characterization of Mycobacterium tuberculosis isolates from elephants of Nepal. Tuberculosis 94(3): 287-292, 2014.

Okuyama, M. W., Shimozuru, M. & Tsubota, T.: A genetic method for sex identification of raccoons (Procyon lotor) with using the ZFX and ZFY genes. J. Vet. Med. Sci. 76(5): 773-775, 2014.

Okuyama, M. W., Shimozuru, M., Yanagawa, Y. & Tsubota, T.: Changes in the immunolocalization of steroidogenic enzymes and the androgen receptor in raccoon (Procyon lotor) testes in association with the seasons and spermatogenesis. J. Reprod. Dev. 60(2): 155-161, 2014.

Sasaki, M., Amano, Y., Hayakawa, D., Tsubota, T., Ishikawa, H., Mogoe, T., Ohsumi, S., Tetsuka, M., Miyamoto, A., Fukui, Y., Budipitojo, T. & Kitamura, N.: Areolae of the placenta in the Antarctic Minke whale (Balaenoptera bonaerensis). J. Reprod. Dev. 60(1): 62-67, 2014.

Saengtienchai, A., Ikenaka, Y., Nakayama, S., Mizukawa, H., Kakehi, M., Bortey-Sam, N., Wageh, D., Tsubota, T., Terasaki, M., Poapolathep, A., Ishizuka, M.: Identification of inter-specific differences in phase II reactions: Determination of metabolites in the urine of 16 mammalian species exposed to environmental pyrene. Environmental Toxicology and Chemistry 33(9): 2062-2069, 2014.

Lee K., Takano, A., Taylor, K., Sashika, M., Shimozuru, M., Konnai, S., Kawabata, H. & Tsubota, T.: A relapsing fever group Borrelia sp. Similar to Borrelia lonestari found among wild sika deer (Cervus Nippon yesoensis) and Haemaphysalis spp. ticks in Hokkaido, Japan. Ticks and Tick-borne Diseasea 5: 841-847, 2014.

Yanagawa, Y., Matsuura, Y., Suzuki, M., Saga, S., Okuyama, H., Fukui, D., Bando, G., Nagano, M., Katagiri, S., Takahashi, Y. & Tsubota, T.: Accessory corpora lutea formation in pregnant Hokkaido sika deer (Cervus nippon) investigated by examination of ovarian dynamics and steroid hormone concentrations. J. Reprod. Dev. 61: 61-66, 2015.

Yamaguchi E, Sashika M, Fujii K, Kobayashi K, Bui VN, Ogawa H, Imai K. Prevalence of multiple subtypes of influenza A virus in Japanese wild raccoons. Virus Research. 189: 8-13, 2014.

Ybañez AP, Sashika M, Inokuma H. The phylogenetic position of Anaplasma bovis, and inferences on the phylogeny of the Genus Anaplasma. Journal of Veterinary Medical Science. 76(2): 307-312. 2014.

 

 

2013年

Original papers

Kooriyama, T., Okamoto, M., Yoshida, T., Nishida, T., Tsubota, T., Saito, A., Tomonaga, M., Matsuzawa, T., Akari H., Nishimura, H. & Miyabe-Nishiwaki, T.: Epidemiological study of zoonoses derived from humans in captive chimpanzees. Primates 54(1): 89-98, 2013. DOI 10.1007/s10329-012-0320-8

Taylor, K. R., Takano, A., Konnai, S., Shimozuru, M., Kawabata, H. & Tsubota, T.: Borrelia miyamotoi infection among wild rodents show age and month independence and correlation with Ixodes persulcatus larval attachment in Hokkaido, Japan. Vector-Borne and Zoonotic Diseases 13 (2): 92-97, 2013.

Murase, Y., Konnai, S., Githaka, N., Hidano, A., Taylor, K., Ito, T., Takano, A., Ando, S., Kawabata, H., Tsubota, T., Murata, S. & Ohashi, K.: Prevalence of Lyme borrelia in Ixodes persulcatus ticks from an area with a confirmed case of Lyme disease. J. Vet. Med. Sci., in press.

Sasaki, M., Amano, Y., Hayakawa, D., Tsubota, T., Ishikawa, H., Mogoe, T., Ohsumi, S., Tetsuka, M., Miyamoto, A., Fukui, Y., Budipitojo, T. & Kitamura, N.: Structure and Steroidogenesis of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis). J. Reprod. Dev. 59(2): 159-167, 2013.

Shimozuru, M., Iibuchi, R., Yoshimoto, T., Nagashima, A., Tanaka, J. & Tsubota, T.: Pregnancy during hibernation in Japanese black bears: effects on body temperature and blood biochemical profiles. J. Mammal. 94(3): 618-627, 2013. DOI: 10.1644/12-MAMM-A-246.1

Taylor, K. R., Takano, A., Konnai, S., Shimozuru, M., Kawabata, H. & Tsubota, T.: Differential tick burdens may explain differential Borrelia afzelii and Borrelia garinii infection rates among four, wild, rodent species in Hokkaido, Japan. J. Vet. Med. Sci. 75(6): 785-790, 2013. DOI: 10.1292/jvms.12-0439

坪田敏男:クマの生息動向と最近の被害状況.日本獣医師会雑誌 66: 131-137, 2013.

Moustafa, M., Ismail, M., Mohamed, A., Ali, A. & Tsubota, T.: Hematological and biochemical parameters of free ranging, female Nile monitors in Egypt. J. Wildl. Dis. 49(3): 750-754, 2013.

Okuyama, W. M., Shimozuru, M., Abe, G., Nakai, M., Sashika, M., Shimada, K-I., Takahashi, N., Fukui, D., Nakamura, R. & Tsubota, T.: Timing of puberty and its relationship with body growth and season in male raccoons (Procyon lotor) in Hokkaido. J. Reprod. Dev. 59(4): 361-367, 2013.

Paudel, S. et al. Molecular characterization of Mycobacterium tuberculosis isolates from elephants of Nepal. Tuberculosis in press.

Okuyama M. W., Shimozuru, M. & Tsubota,T.: A genetic method for sex identification of raccoons (Procyon lotor) with using the ZFX and ZFY genes. J. Vet. Med. Sci., in press.

Okuyama, M. W., Shimozuru, M., Yanagawa, Y. & Tsubota, T.: Changes in the immunolocalization of steroidogenic enzymes and the androgen receptor in reccoon (Procyon lotor) testes in association with the seasons and spermatogenesis. J. Reprod. Dev., in press.

 

 

2012年

Original papers

Kamine, A., Shimozuru, M., Shibata, H. & Tsubota, T.: Effects of tiletamine-zolazepam with and without premedications on blood chemistry and glucose tolerance tests in Japanese black bears (Ursus thibetanus japonicus). American Journal of Veterinary Research 60(1): 5-13, 2012.

Weng, Q., Tsubota, T., Dai, M., Tian, Y., Zhang, H., Xu, M., Watanabe, G. & Taya, K.: Immunolocalization of steroidogenic enzymes and its expression during the breeding season in the testes of the wild raccoon dogs (Nyctereutes procyonoides). Animal Science Journal 83: 535-542, 2012.

Kamine, A., Shimozuru, M., Shibata, H. & Tsubota, T.: Changes in blood glucose and insulin responses to intravenous glucose tolerance tests and blood biochemical values in adult female Japanese black bears (Ursus thibetanus japonicus). Jpn. J. Vet. Res. 60: 5-13, 2012.

Okuyama W. M., Shimozuru, M., Takahashi, N., Fukui, D., Nakamura, R. & Tsubota, T.: Seasonal changes in spermatogenesis and peripheral testosterone concentration in raccoon (Procyon lotor) in Hokkaido. J. Vet. Med. Sci. 74: 727-732, 2012.

條野真奈美,小林由美,浅沼武敏,坪田敏男,桜井泰憲:胎子期におけるトド(Eumetopias jubatus)の成長様式と形態的特徴.北海道大学水産科学研究報 62(1): 1-7, 2012.

Kooriyama, T., Hasegawa, H., Shimozuru, M., Tsubota, T., Nishida, T. & Iwaki, T.: Parasitology of five primates in Mahale Mountains National Park, Tanzania. Primates 53: 365-375, 2012.

Shimozuru, M., Kamine, A. & Tsubota, T.: Changes of hepatic genes involved in lipid metabolism during pre-hibernation period in captive adult female Japanese black bears (Ursus thibetanus japonicus). Can. J. Zool. 90: 945-954, 2012.

Takano, A., Sugimori, C., Fujita, H., Kadosaka, T., Taylor, K. R., Tsubota, T., Konnai, S., Tajima, T., Sato, K., Watanabe, H., Ohnishi, M. & Kawabata, H.: A novel Relapsing fever Borrelia sp. infects the salivary glands of the molted hard-bodied tick, Amblyomma geoemydae. Ticks and Tick-Borne Diseases 3: 259-261, 2012.

高見一利,渡邊有希子,坪田敏男,福井大祐,大沼 学,山本麻衣,村田浩一:野生動物の感染症管理にどのように取り組むべきか.日本野生動物医学会誌 17: 33-42, 2012.

Shimozuru, M., Kamine, A. & Tsubota, T.: Changes in expression of hepatic genes involved in energy metabolism during hibernation in captive, adult, female Japanese black bears (Ursus thibetanus japonicus). Comp. Biochem. Physiol. Part B 163: 254-261, 2012.

 

 

2011年

Original papers

Yamanaka, A., Asano, M., Suzuki, M., Mizoguchi, T., Oi, T., Shimozuru, M. & Tsubota, T.: Evaluation of stored body fat of nuisance-killed Japanese black bears (Ursus thibetanus japonicus). Zool. Sci. 28: 105-111, 2011.

Ogawa, K., Yamaguchi, K., Suzuki, M., Tsubota, T., Ohya, K. & Fukushi, H.: Genetic characteristics and antimicrobial resistance of Escherichia coli from Japanese macaques (Macaca fuscata) in rural Japan. J. Wildl. Dis. 47: 261-270, 2011.

Yamanaka, A., Yamauchi, K., Tsujimoto, T., Tsubota, T., Mizoguchi, T., Oi, T., Sawada, S., Shimozuru, M. & Tsubota, T.: Estimating the success rate of ovulation and early litter loss rate in the Japanese black bear (Ursus thibetanus japonicus) by examining the ovaries and uteri. Jpn. J. Vet. Res. 59: 31-39, 2011.

Takano, A., Nakao, M., Masuzawa, T., Takada, N., Yano, Y., Ishiguro, F., Fujita, H., Ito, T., Ma, X., Oikawa, Y., Kawamori, F., Kumagai, K., Mikami, T., Hanaoka, N., Ando, S., Honda, N., Taylor, K., Tsubota, T., Konnai, S., Watanabe, H., Ohnishi, M. & Kawabata, H.: Multi-locus sequence typing of Borrelia garinii isolated from human, ticks, and rodents; Rodents were implicated as the main reservoir host of human pathogenic B. garinii in Japan. J. Clin. Microbiol. 49: 2035-2039, 2011.

Nakashita, R., Suzuki, Y., Akamatsu, F., Sato-Hashimoto, M. & Tsubota, T.: Ecological application of compound-specific stable nitrogen isotope analysis of amino acids - A case study for captive and wild bears. Researches in Organic Geochemistry 27: 73-79, 2011.

 

 

2010年

Original papers

Hayakawa, D., Sasaki, M., Suzuki, M., Tsubota, T., Igota, H. & Kitamura, N.: Immunohistochemical localization of steroidogenic enzymes in the testis of the sika deer (Cervus nippon) during developmental and seasonal changes. J. Reprod. Dev. 56: 117-123, 2010.

Iibuchi, R., Kamine, A., Shimozuru, M., Nio-Kobayashi, J., Watanabe, G., Taya, K. & Tsubota, T.: Changes in plasma gonadotropins, inhibin and testosterone concentrations and testicular gonadotropin receptor mRNA expression during testicular active, regressive and recrudescent phase in the captive Japanese black bear (Ursus thibetanus japonicus). Jpn. J. Vet. Res. 57: 185-196, 2010.

Nakagawa, E,, Kobayashi, M., Suzuki, M. & Tsubota, T.: Genetic variation in the harbor seal Phoca vitulina and the spotted seal Phoca largha in Hokkaido, based on mitochondrial DNA cytochrome b sequences. Zool. Sci. 27: 263-268, 2010.

Iibuchi, R.,Shimozuru, M., Kamine, A., Nio-Kobayashi, J., Iwanaga, T. & Tsubota, T.: Localization of five steroidogenic enzyme mRNAs in Japanese black bear (Ursus thibetanus japonicus) testes during the mating season by in situ hybridization. J. Reprod. Dev. 56: 236-242, 2010.

久保正仁,千葉依里,飯渕るり子,坪田敏男,佐々木基樹,酒井洋樹,柳井徳磨:エゾヒグマ(Ursus arctos yesoensis)におけるHepatozoon sp.寄生について. 日本野生動物医学会誌 15: 111-113, 2010.

Adachi, I., Kusuda, S., Nagao, E., Taira, Y., Asano, M., Tsubota, T. & Doi, O. Fecal steroid metabolites and reproductive monitoring in female Tsushima leopard cat (Prionailurus bengalensis euptilurus). Theriogenology 74: 1499-1503, 2010.

 

 

2009年

Original papers

Nakamura, S., Nishii, N., Yamanaka, A., Kitagawa, H., Asano, M., Tsubota, T. & Suzuki, M.: Leptin receptor (Ob-R) expression in the ovary and uterus of wild Japanese black bears (Ursus thibetanus japonicus) J. Reprod. Dev. 55: 110-115, 2009.

Yanagawa, Y., Matsuura, Y., Suzuki, M., Saga, S., Okuyama, H., Fukui, D., Bandou, G., Katagiri, S., Takahashi, Y. & Tsubota, T.: Fetal age estimation of Hokkaido Sika deer (Cervus nippon yesoensis) using ultrasonography during early pregnancy. J. Reprod. Dev. 55: 143-148, 2009.

Okano, T., Murase, T., Nakamaura, S., Komatsu, T., Tsubota, T., Asano, M. & Suzuki, M.: Normal sperm morphology and changes of semen characteristics and abnormal morphological spermatozoa among peri-mating seasons in captive Japanese black bears (Ursus thibetanus japonicus). J. Reprod. Dev. 55: 194-199, 2009.

Iibuchi, R., Nakano, N., Nakamura, T., Urashima, T., Shimozuru, M., Murase, T. & Tsubota, T.: Change in body weight of mothers and neonates and in milk composition during denning period in captive Japanese black bears (Ursus thibetanus japonicus). Jpn. J. Vet. Res. 57: 13-22, 2009.

Kubo, M., Okano, T., Tsubota, T., Sakai, H. & Yanai, T.: Muscular sarcocystosis in wild carnivores in Honshu, Japan. Parasitol. Res. 106: 213-219, 2009.

Nakagawa, E., Kobayashi, M., Suzuki, M. & Tsubota, T.: Growth variation in skull morphology of Kuril harbor seals (Phoca vitulina stejnegeri) and spotted seals (Phoca largha) in Hokkaido, Japan. Jpn. J. Vet. Res. 57: 147-162, 2009.

Yamanaka, A., Asano, M., Suzuki, M., Mizoguchi, T., Shimozuru, M. & Tsubota, T.: Is there any relationship between the number of nuisance-killed Japanese black bears (Ursus thibetanus japonicus) and their nutritional condition? Biology of Bear Intrusions: 48-51, 2009.

Tsubota, T., Nakamura, S., Suzuki, M., Kamine, A. & Shimozuru, M.: Physiological and endocrinological mechanism for body fat accumulation during the pre-hibernation period in the captive Japanese black bear, Ursus thibetanus japonicus. Biology of Bear Intrusions: 52-55, 2009.

The laboratory's ultimate goal is biodiversity conservation.

Gruaduate School of Veterinary Medicine